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A LOCAL‐LESION TECHNIQUE FOR MEASURING THE INFECTIVITY OF CONIDIA OF BOTRYTIS FABAE SARDIÑA
Author(s) -
LAST F. T.,
HAMLEY ROSEMARY E.
Publication year - 1956
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1956.tb02135.x
Subject(s) - biology , conidium , vicia faba , botany , horticulture , inoculation , lesion , botrytis cinerea , infectivity , psychology , virus , virology , psychiatry
Discrete lesions developed when conidial suspensions of Botrytis fabae Sardiña were rubbed with the forefinger on leaves of broad bean plants ( Vicia faba L.) subsequently kept in a water‐saturated atmosphere. The numbers of lesions which developed were directly proportional to the concentration of inoculum. At equal concentrations, conidia from young cultures produced more lesions than conidia from old cultures. Variation in lesion numbers between plants within a pot always considerably exceeded the variation between opposite half‐leaflets of a leaf, as did variation between pots and between leaves of old, but not of young plants. As the standard error of the number of lesions developing per half‐leaflet ( x ) increases as the mean increases, the values of x need to be transformed before being statistically analysed. A suitable transformation is z = log 10 1/2{x + c +√(x 2 + 2cx)}, where c = 20.