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FURTHER STUDIES ON THE BREAKDOWN OF 2:4‐DICHLOROPHENOXYACETIC ACID BY A SOIL BACTERIUM
Author(s) -
AUDUS L. J.,
SYMONDS K. V.
Publication year - 1955
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1955.tb02423.x
Subject(s) - 2,4 dichlorophenoxyacetic acid , biology , bacteria , bacterial growth , detoxication , toxicity , kinetics , chromatography , botany , biochemistry , organic chemistry , enzyme , chemistry , genetics , physics , quantum mechanics
Bacterial growth rates and the kinetics of detoxication have been followed during the course of breakdown of 2:4‐dichlorophenoxyacetic acid in a liquid mineral salt medium by cultures of Bacterium globiforme isolated from garden soil. It was found that toxicity persisted apparently unchanged long after bacterial growth had ceased, and finally disappeared suddenly after a lag period comparable in duration with those obtained in soil perfusion experiments. These results can best be explained by the production of highly phytotoxic intermediates in the first stages of 2:4‐D breakdown. Preliminary experiments involving paper partition chromatography indicate that there may be at least two such intermediates.