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INVESTIGATION INTO THE PRODUCTION OF BACTERIOSTATIC SUBSTANCES BY FUNGI A REVISION OF THE TESTING METHOD
Author(s) -
WILKINS W. H.
Publication year - 1949
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1949.tb06416.x
Subject(s) - biology , nutrient agar , dilution , fungus , agar , mycelium , food science , biological system , microbiology and biotechnology , botany , bacteria , genetics , physics , thermodynamics
Discrepancies in the comparative results of workers on antibacterial substances produced by fungi can usually be attributed to the use of different testing methods. The method described here for testing fungus metabolism solutions is designed to facilitate comparison in that its characteristic features are simplicity and rapidity. A nutrient broth of the standard ingredients of peptone, salt and water only, plus agar, ensures that a test involving IOO plates, i.e. 400 tests, can be set up by one assistant in 3 hr. The sensitivity of the test is almost equal to that of a dilution series and the results are consistent over a wide range of individual and circumstantial variation.