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The Formation Of Intracellular Inclusions In Solanaceous Hosts Infected With Aucuba Mosaic Of Tomato 1
Author(s) -
SHEFFIELD F. M. L.
Publication year - 1931
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1931.tb02319.x
Subject(s) - cytoplasm , biology , nucleus , plasmodesma , solanum , protoplasm , cytoplasmic inclusion , nicotiana tabacum , intracellular , cytoplasmic streaming , biophysics , microbiology and biotechnology , botany , genetics , gene
S ummary A description is given of the mode of formation of intracellular inclusions produced by aucuba mosaic of tomato in Solanum nigrum, S. nodiflorum, S. lycopersicum, Nicotiana tabacum and Hyoscyamus niger. Soon after infection the rate of streaming of the cytoplasm is increased, then minute particles of protein appear in the cytoplasm which carries them passively about the cell. These particles aggregate and fuse to form large masses which are still carried passively but more slowly about the cell. These fuse until all the protein material is contained in one or occasionally more granular masses. In the three Solanum species examined this mass becomes rounded and it may lose its granular appearance and become vacuolated. In N. tabacum the body does not always round off and in H. nip it very seldom does so but remains as an irregularly shaped granular mass which may, however, become vacuolate. There is no evidence at any time of autonomous movement, the particles and the fully formed body being carried, as are the cell nucleus, mitochondria, etc., of the normal plant, in the cytoplasmic stream. After the spherical body is formed spike‐like crystal appears in the cell. The cell remains at rest for the space of several weeks. Often the rounded inclusion body and the nucleus are juxtaposed, but there is no special significance in this, it is merely the accidental result of the mode of formation of the body. Particles tend to accumulate where number of strands of plasm meet; usually several strands converge on the nucleus. Ultimately the body breaks down giving number of protein crystals. After some months these dissolve. In H. niger the inclusion bodies are confined to the chlorotic areas where they are abundant in all tissues. In the other species studied they are distributed over green and yellow tissues. They are very abundant in the hairs, less so in the epidermis and very rare in the palisade and spongy tissues. In H. niger the development of the palisade tissue is arrested, in the other species the development is not so obviously affected although growth is retarded. These inclusions appear not to be organismal in nature; they seem to be products of reaction of the host cell to the virus, but they may contain the etiological agent of the disease.