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SOME OBSERVATIONS ON THE GROWTH OF RHIZOCTONIA CROCORUM ( PERS.) DC. IN PURE CULTURE
Author(s) -
BUDDIN W.,
WAKEFIELD E. M.
Publication year - 1924
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1924.tb05711.x
Subject(s) - biology , rhizoctonia , organism , isolation (microbiology) , spore , agar , inoculation , fungus , botany , microbiology and biotechnology , horticulture , bacteria , rhizoctonia solani , paleontology , genetics
SUMMARY. The organism dealt with in this paper is that which has been commonly known in this country as Rhizoctonia violacea Tul. but until satisfactory proof is forthcoming of a spore stage of the organism it should be known by its valid prior name, Rhizoctonia Crocorum (Pers.) DC. The methods used for isolation of the fungus in pure culture are described and some account given of preliminary inoculation experiments with the re‐isolation of the parasite. It is shown that growth of R. Crocorum will occur on a very wide range of sterilised artificial material when in pure culture. No spore form has occurred in any of the large number of cultures which have been grown. Some notes are given on difficulties arising in temperature relation work from differences in the “internal condition” of the organism under varying conditions, and it is shown that the optimum temperature for the growth of the organism in culture on meat‐malt extract agar as measured by the increase in diameter of colonies is in the neighbourhood of 25.5° C. This optimum temperature probably explains the geographical distribution of the severe attacks of the organism in the field.