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Analysis of genetic diversity in the bioenergy plant E rianthus arundinaceus ( P oaceae: A ndropogoneae) using amplified fragment length polymorphism markers
Author(s) -
Tsuruta Shinichi,
Ebina Masumi,
Kobayashi Makoto,
Hattori Taiichiro,
Terauchi Takayoshi
Publication year - 2012
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/j.1744-697x.2012.00258.x
Subject(s) - amplified fragment length polymorphism , biology , genetic diversity , primer (cosmetics) , polymorphism (computer science) , genetics , microbiology and biotechnology , genotype , gene , chemistry , population , demography , organic chemistry , sociology
The genetic diversity among 30 accessions including 18 E rianthus arundinaceus and 12 S accharum species was analyzed using amplified fragment length polymorphism ( AFLP ) markers. We obtained 2245 fragments using 16 primer combinations. The E rianthus collection generated 1115 fragments of which 772 (69.2%) were polymorphic. Of the 342 monomorphic fragments (30.8%), 222 were specific to E . arundinaceus . The neighbor‐joining tree based on the detected fragments clearly separated the E rianthus and S accharum accessions into distinct groups. The E rianthus collection was further subdivided into two J apanese groups and one I ndonesian group, with a high level of genetic differentiation among these groups ( F st = 0.2062). These results represent the first molecular characterization of the J apanese E . arundinaceus breeding materials and provide a useful initial resource for breeding to improve the bioenergy potential of E rianthus spp.