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Multiple shoot formation, somatic embryogenesis and plant regeneration from seed‐derived shoot apical meristems in ruzigrass ( Brachiaria ruziziensis )
Author(s) -
Ishigaki Genki,
Gondo Takahiro,
Suenaga Kazuhiro,
Akashi Ryo
Publication year - 2009
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/j.1744-697x.2009.00137.x
Subject(s) - shoot , somatic embryogenesis , biology , kinetin , botany , germination , explant culture , gibberellic acid , murashige and skoog medium , meristem , micropropagation , in vitro , biochemistry
We have established a plant regeneration system via multiple shoot formation, or somatic embryogenesis from seed‐derived shoot apical meristems of ruzigrass ( Brachiaria ruziziensis ). Shoot apices as initial explants were isolated from aseptically germinated seedlings, and cultured in vitro . The most effective treatments for multiple shoots and embryogenic calli formation were 0.5 mg L −1 2,4‐dichlorophnoxyacetic acid (2,4‐D) plus 2.0 mg L −1 6‐benzylaminopurine (BAP) and 4.0 mg L −1 2,4‐D plus 0.2 mg L −1 BAP, respectively. Plant regeneration from multiple shoot‐clumps was achieved by culturing on solid MS medium containing 1.0 mg L −1 BAP or 2.0 mg L −1 kinetin plus 2.0 mg L −1 gibberellic acid. Somatic embryos regenerated plantlets on a MS medium without plant hormones. Regenerated shoots were germinated precociously on MS medium containing 0.01 or 0.1 mg L −1 naphthalene acetic acid and 2.0 mg L −1 BAP. All regenerants were successfully grown up to maturity. The plant regeneration via multiple‐shoot clumps and embryogenic calli formation can be used as an alternative regenerable target tissue for genetic transformation using particle bombardment of ruzigrass.