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Development and characterization of simple sequence repeat markers in Zoysia japonica Steud.
Author(s) -
Tsuruta ShinIchi,
Hashiguchi Masatsugu,
Ebina Masumi,
Matsuo Taiyo,
Yamamoto Toshiya,
Kobayashi Makoto,
Takahara Manabu,
Nakagawa Hitoshi,
Akashi Ryo
Publication year - 2005
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/j.1744-697x.2005.00033.x
Subject(s) - zoysia japonica , biology , microsatellite , genetics , primer (cosmetics) , genetic diversity , genetic marker , genetic analysis , genetic linkage , allele , botany , gene , population , chemistry , demography , organic chemistry , sociology
Simple sequence repeats (SSR) have several positive attributes as genetic markers when compared with other DNA‐based marker systems. Because of their successful use for genetic analysis in a number of plant species, they could be similarly useful tools for the genetic study of various Zoysia species. A genomic library enriched for the AG/TC motif was developed from Zoysia japonica cv. ‘Asagake’ and screened by using 5′‐biotin‐labeled oligonucleotides (AG 20 ). As a result of sequencing analysis of 162 clones, a total of 119 clones were identified containing 8–35 SSR repeats. Thirty‐two primer pairs designed from these clones were evaluated for their ability to detect polymorphisms among six additional zoysiagrass cultivars. The average values of the observed heterozygosities and polymorphic information content for the individual loci were 0.57 and 0.69, respectively. These primers were successful in generating several informative cross‐amplification products in additional Zoysia species. The generation of informative SSR markers should be a valuable tool for identification, estimation of genetic diversity and construction of genetic linkage maps in Zoysia species.