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Introduction of exogenous substance into protoplasts of perennial ryegrass by electroporation
Author(s) -
Unno Hiroki,
Yamamoto Shinrô
Publication year - 2005
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/j.1744-697x.2005.00025.x
Subject(s) - fructan , protoplast , electroporation , perennial plant , botany , cellulase , chemistry , biology , food science , biochemistry , cellulose , sucrose , gene
Involvement of a substance in the tolerance of plants will be clarified by increasing the substance in the plants and detecting the effect on the tolerance. Some substances, however, are difficult to be increased in intact plants. Accordingly, the introduction of an exogenous substance into protoplasts of grass was investigated by means of electroporation using fructan as the exogenous substance. Protoplasts were freshly prepared from young leaves of perennial ryegrass by treating with 2% cellulase. Fifty percent of the protoplasts were alive after electroporation at 500 V/cm at 18 µF. Fructan concentration in the protoplasts was increased by electroporation as described above in the presence of exogenous fructan and the increase in the fructan was promoted with an increase in concentration of the exogenous fructan. The results obtained suggest that concentrations of substances in grass protoplasts are able to be increased by electroporation.