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Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep
Author(s) -
Dubey P. K.,
Goyal S.,
Kathiravan P.,
Mishra B. P.,
Gahlawat S. K.,
Kataria R. S.
Publication year - 2013
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2012.01135.x
Subject(s) - biology , phylogenetic tree , gene , vertebrate , phylogenetics , genetics , evolutionary biology
Summary The present study was undertaken to characterize the full‐length transcripts of Toll‐like receptor (TLR) genes 1–10 of river buffalo. The conceptualized amino acid identity of bubaline TLR s ranged between 86% to 100% with ruminants, while it ranged between 45% to 91% with other vertebrate species. Simple modular architecture tool ( SMART ) analysis revealed the presence of TIR domains and varying numbers of leucine‐rich repeat motifs in all the buffalo TLR s. With respect to TIR domains, TLR s 1, 2 and 3 of river buffalo were found to have 99.3% identity with cattle and 100% identity of TLR s 4, 6 and 10 with sheep. Phylogenetic analysis of TLR s of buffalo and different vertebrate species revealed the clustering of major TLR gene subfamilies with high bootstrap values. The evolutionary relationship between buffalo and other ruminant species was found to vary among different TLR s. In order to understand the relationship between TLR s of different ruminant species, multidimensional scaling ( MDS ) analysis of pairwise amino acid differences between different species within each TLR was performed. Buffalo and cattle were found to be closely related only with respect to TLR s 1, 2 and 7, while buffalo and sheep were found to be clustering together with respect to TLR s 3, 6, 8 and 10. The distinct relationship of bubaline TLR s with cattle and sheep revealed the possible differences in the pathogen recognition receptor systems in these animals and consequently the differences in their susceptibility/resistance to various invading organisms.