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Detection of two HLA‐B*27 alleles, B*27:25 and B*27:86, in two Taiwanese blood donors by sequence‐based typing
Author(s) -
Yang E. K. L.,
Lee S. K.,
Lin P. Y.
Publication year - 2012
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2011.01081.x
Subject(s) - exon , hla b , amino acid substitution , nucleotide , genetics , haplotype , biology , microbiology and biotechnology , point mutation , allele , sequence (biology) , nucleic acid sequence , peptide sequence , mutation , gene
Summary We report here two HLA‐B*27 alleles, B*27:86 and B*27:25, found in two Taiwanese blood donors. The new sequence of B*27:86 is identical to B*27:04:01 in exons 2 and 3, except at nucleotide 602 (A→G) in exon 3. The nucleotide change caused an amino acid substitution from E to G at amino acid residue 177. The sequence of B*27:25 is identical to B*27:04:01 in exons 2, 3, 4, 5, 6 and 7 except at nucleotides 538, 539, 559 and 560 in exon 4. The nucleotide changes caused amino acid substitutions from L to W and from E to L at residues 156 and 163, respectively. The generation of B*27:86 was probably resulted by a point mutation while the generation of B*27:25 may have been derived from a sequence recombination event between B*46:01:01 and B*27:04:01. The probable HLA‐A, ‐B and ‐DRB1 haplotypes in association with B*27:86 and B*27:25 may be deduced as A*11:53‐B*27:86‐DRB1*12 and A*11:01‐B*27:25‐DRB1*04:05, respectively.