Premium
IL‐6 SNP diversity among four ethnic groups as revealed by bead‐based liquid array profiling
Author(s) -
Ivanova M.,
Ruiqing J.,
Kawai S.,
Matsushita M.,
Ochiai N.,
Maruya E.,
Saji H.
Publication year - 2011
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2010.00972.x
Subject(s) - bead , snp , profiling (computer programming) , ethnic group , diversity (politics) , magnetic bead , biology , computational biology , evolutionary biology , single nucleotide polymorphism , genetics , chromatography , geography , chemistry , genotype , computer science , sociology , gene , archaeology , anthropology , operating system
Summary In the present work, we established a rapid, cost‐effective and high‐throughput method for genotyping using a multiplexed microsphere‐based suspension array platform – Luminex ® × MAP™, which enabled us to analyse two SNPs in the promoter of IL‐6 gene, determining haplotypes associated with different levels of expression. Using this system, IL‐6 diversity in four different ethnic groups – East Asians, Caucasians, Hispanic and African Americans – was assessed. Results showed a significant variability in terms of allele, genotype and haplotype distribution. Considering the important immunoregulatory role of this cytokine and as a clinically relevant marker, this genotyping approach will provide a powerful tool for disease association or transplant outcome studies.