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Interleukin‐10 gene promoter polymorphism in Polish rheumatoid arthritis patients
Author(s) -
ParadowskaGorycka A.,
Trefler J.,
MaciejewskaStelmach J.,
Łącki J. K.
Publication year - 2010
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2010.00913.x
Subject(s) - genotype , erythrocyte sedimentation rate , rheumatoid arthritis , medicine , gastroenterology , allele frequency , polymorphism (computer science) , allele , arthritis , genotype frequency , interleukin 6 , immunology , gene , biology , cytokine , genetics
Summary Interleukin (IL)‐10 is an important multifunctional cytokine with both anti‐inflammatory and immunoregulatory effects in rheumatoid arthritis (RA). In the present study, we evaluated the frequency and potential impact of IL‐10 promoter polymorphisms on susceptibility to and severity of RA in Polish in – patients with a high disease activity (mean DAS 28 C‐reactive protein 5.25). DNA was obtained from 244 RA patients and 106 healthy controls. The −592C/A and −1082G/A IL‐10 gene polymorphisms were amplified by polymerase chain reaction with restriction endonuclease mapping. The frequency of the IL‐10‐592CA, ‐592AA genotypes (respectively: 30% vs 5% and 7% vs 0%) and allele −592A (37% vs 5%) were significantly higher in RA patients as compared with a control group. We did not find any association of the IL‐10‐592C/A genotype distribution with disease parameters, except for an increased ESR (erythrocyte sedimentation rate) in patients with the −592CC genotype as compared with those with −592CA or −592AA genotypes ( P = 0.01). The frequency of the IL‐10‐1082GG genotype was lower ( P = 0.0001), and that of the IL‐10‐1082GA genotype was higher ( P = 0.009) in RA patients comparing with the control group. In RA patients with −1082GA or −1082AA genotypes the time duration of the disease ( P = 0.03), Health Assessment Questionnaire (HAQ) Score ( P = 0.04) and PLT count ( P = 0.001) were significantly increased as compared with subjects with −1082GG genotype. Presented findings indicate that IL‐10‐592C/A and IL‐10‐1082G/A polymorphisms may be considered genetic risk factors for RA susceptibility and severity.