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The mutations of Th1 cell‐specific T‐box transcription factor may be associated with a predominant Th2 phenotype in gastric cancers
Author(s) -
Yang P.,
Qiu G.,
Wang S.,
Su Z.,
Chen J.,
Wang S.,
Kong F.,
Lu L.,
Ezaki T.,
Xu H.
Publication year - 2010
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2010.00899.x
Subject(s) - biology , phenotype , transcription factor , gene , cancer , transfection , peripheral blood mononuclear cell , immune system , cancer research , genetics , microbiology and biotechnology , in vitro
Summary Gastric cancer is a serious public health cancer and causes nearly 1 million deaths a year worldwide. Th1 cells play critical roles in orchestrating the adaptive immune responses against gastric cancer. T‐bet , a member of the T‐box family of transcription factors, is the Th1 master regulator and up‐regulated during Th1 differentiation. Polymorphisms have also been shown to exist in T‐bet. Some reports indicated that some tumours were associated with the drift of Th1 and Th2. In the present work, we investigated the drift of Th1/Th2 by detecting the expression levels of T‐bet , IFN‐γ, IL‐4, and GATA‐3 in peripheral blood mononuclear cell of gastric cancer patients by real‐time PCR, explored the relationship between the polymorphism of T‐bet gene and drift of Th1/Th2 by gene sequence, western blot, and gene transfection. Our results indicated that a predominant Th2 phenotype was existence. T‐bet gene mutations may be associated with Th2‐dominated condition in gastric cancers.