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Sequence‐based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines
Author(s) -
Ho C. S.,
FranzoRomain M. H.,
Lee Y. J.,
Lee J. H.,
Smith D. M.
Publication year - 2009
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2009.00853.x
Subject(s) - biology , allele , xenotransplantation , gene , typing , antigen , immune system , genetics , transplantation , cell culture , microbiology and biotechnology , virology , medicine , surgery
Summary A total of 53 alleles at five highly polymorphic swine leucocyte antigen (SLA) loci (SLA‐1, SLA‐3, SLA‐2, SLA‐DRB1, and SLA‐DQB1) were identified in eight commercially available porcine cell lines (ESK‐4, LLC‐PK1, MPK, PK13, PK15, PT‐K75, SK‐RST, and ST). This information is essential for the use of these cell lines to understand the role of SLA genes and proteins in swine models of transplantation, xenotransplantation, and in swine immune responses to infectious diseases and vaccines. The ready availability of these cell lines also makes them a good source of reference DNA for SLA allele typing.