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Influence of HLA‐DR2 on perforin‐positive cells in pulmonary tuberculosis
Author(s) -
Rajeswari D. N.,
Selvaraj P.,
Raghavan S.,
Jawahar M. S.,
Narayanan P. R.
Publication year - 2007
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2007.00703.x
Subject(s) - perforin , cytotoxic t cell , human leukocyte antigen , cd8 , immunology , medicine , flow cytometry , biology , immune system , antigen , genetics , in vitro
Summary Perforin is one of the key effector molecules of cytotoxic T cells and natural killer cells. The influence of HLA‐DRB1 alleles on peripheral blood perforin‐positive CD4, CD8, CD16 and CD56 cells was studied by flow cytometry. HLA‐DRB1 typing was done in normal healthy subjects (NHS: n = 156) and patients with pulmonary tuberculosis (PTB: n = 102) by polymerase chain reaction‐based sequence‐specific oligonucleotide hybridization method. We observed a significantly decreased percentage of total perforin‐positive cells (per + ) ( P = 0.0004); CD8 + /Per + ( P = 0.0005); CD16 + /Per + ( P = 0.05) and CD56 + /Per + cells ( P = 0.001) in HLA‐DR2‐positive PTB patients compared to non‐DR2 patients. Subtyping of HLA‐DR2‐positive subjects at the allelic level revealed that the percentage of CD8 + /Per + cells did not differ among DRB1*1501 and DRB1*1502 patients while a trend towards a decreased percentage of CD16 + /Per + and CD56 + /Per + cells was noticed in DRB1*1501 patients compared to DRB1*1502 patients. The present study suggests that HLA‐DR2 may be associated with down‐regulation of perforin‐positive cytotoxic lymphocytes and natural killer cells in pulmonary tuberculosis.