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No association of SUMO4 M55V with autoimmune diabetes in Asian‐Indian patients
Author(s) -
Sedimbi S. K.,
Kanungo A.,
Shastry A.,
Park Y.,
Sanjeevi C. B.
Publication year - 2007
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2007.00668.x
Subject(s) - diabetes mellitus , genetic predisposition , medicine , restriction fragment length polymorphism , allele , autoimmune diabetes , gastroenterology , type 1 diabetes , polymerase chain reaction , genetics , biology , gene , endocrinology , disease
Summary Autoimmune diabetes [type 1 diabetes mellitus (T1DM), latent autoimmune diabetes in adults (LADA) and part of malnutrition‐related diabetes] has been shown to have genetic predisposition. Studies in IDDM 5 have lead to the discovery of a novel polymorphism 163 A→G, of SUMO4 (small ubiquitin‐related modifier) gene, associated with risk to T1DM in Asians, but not in Caucasians. We studied patients with T1DM ( n = 134), patients with LADA ( n = 101), patients with malnutrition‐modulated diabetes mellitus ( n = 66) and patients with fibrocalculous pancreatic diabetes ( n = 43) and healthy controls subjects ( n = 114) from Cuttack, India. Polymerase chain reaction–sequence‐specific primer (PCR‐SSP) was used to amplify the 163 A→G sequences. Restriction fragment length polymorphism (RFLP) was performed using restriction enzyme Taq I (PCR‐RFLP). Differences in the allelic frequencies of the A and the G alleles were tested statistically using Fisher's exact test or chi‐squared test wherever appropriate. P ‐values were considered significant when equal to or less than 0.05. No significant association was detected between SUMO4 M55V and T1DM susceptibility in Asian‐Indians. Comparison of the A and G alleles with HLA DR3‐DR4 did not result in any significant P ‐values. No significant association was found between SUMO4 M55V and LADA or malnutrition‐related diabetes mellitus (MRDM). Our results show that Asian‐Indians with T1DM are different from other Asian populations. Asian‐Indians show more similarity to Caucasians with respect to the association of SUMO4 M55V variant in T1DM. Association studies on Asian‐Indian patients with LADA and MRDM showed no significant difference in the presence of the A and the G alleles when compared to healthy controls.