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Molecular cloning and sequencing of MHC class II beta 1 domain of turkey reveals high sequence identity with chicken
Author(s) -
Ahmed K. A.,
Saxena V. K.,
Saxena M.,
Ara A.,
Pramod A. B.,
Rajaram M. L.,
Dorman K. S.,
Majumdar S.,
Rasool T. J.
Publication year - 2007
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2007.00661.x
Subject(s) - biology , genetics , major histocompatibility complex , restriction fragment length polymorphism , mhc class i , haplotype , restriction enzyme , restriction site , mhc class ii , phylogenetic tree , exon , genotyping , gene , polymerase chain reaction , genotype
Summary We report the nucleotide sequences of turkey ( Meleagris gallopavo ) major histocompatibility complex (MHC) class II loci (β 1 domain or exon 2 encoding the peptide‐binding region). In the present investigation, three distinct sequences from the β 1 domain of turkey MHC class II were isolated. A BLAST search and phylogenetic analysis revealed that turkey MHC sequences are most similar to chicken and peacock MHC. There was no strong evidence of recombination among the turkey MHC sequences or with other avian MHC, but diversity was high. The diversity in this peptide‐binding region may be the result of point mutation and balancing selection or frequent gene conversion within turkey. However, more work and data are needed to understand the evolution of turkey and other avian MHC. Moreover, polymerase chain reaction–restriction fragment‐length polymorphism analysis of exon 2 using the Hinf I restriction enzyme demonstrated three restriction patterns and a preliminary evidence of multiple β loci in turkey. PCR‐RFLP analysis of turkey MHC class II loci could be a promising method of MHC genotyping, when more sequences are available. Turkey MHC haplotypes identified earlier by RFLP analysis should be sequenced to standardize turkey MHC nomenclature and to develop DNA based method of haplotyping.