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SNPs in human β‐defensin 1 gene ( DEFB1 ): frequencies in a Mexican population and new PCR–RFLPs assays
Author(s) -
PradoMontes de Oca E.,
RangelVillalobos H.,
GallegosArreola M. P.,
Sandoval L.,
Figuera L. E.
Publication year - 2006
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2006.00628.x
Subject(s) - genotyping , restriction fragment length polymorphism , single nucleotide polymorphism , biology , genetics , polymerase chain reaction , allele , allele frequency , pyrosequencing , polymorphism (computer science) , defensin , gene , microbiology and biotechnology , genotype
Summary Polymerase chain reaction–restriction fragment length polymorphism assays for two single nucleotide polymorphisms in the human β‐defensin 1 gene have been validated with real‐time PCR in 101 healthy individuals from western Mexico. Allele frequencies were 52.5% (692‐A) and 98.5% (1836‐A). These assays can be confidently used as a cheaper alternative genotyping method for these sites.