SNPs in human β‐defensin 1 gene ( DEFB1 ): frequencies in a Mexican population and new PCR–RFLPs assays | Zendy

PradoMontes de Oca E. | Zendy; RangelVillalobos H. | Zendy; GallegosArreola M. P. | Zendy; Sandoval L. | Zendy; Figuera L. E. | Zendy

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SNPs in human β‐defensin 1 gene ( DEFB1 ): frequencies in a Mexican population and new PCR–RFLPs assays

Author(s) -

PradoMontes de Oca E.,

RangelVillalobos H.,

GallegosArreola M. P.,

Sandoval L.,

Figuera L. E.

Publication year - 2006

Publication title -

international journal of immunogenetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.41

H-Index - 47

eISSN - 1744-313X

pISSN - 1744-3121

DOI - 10.1111/j.1744-313x.2006.00628.x

Subject(s) - genotyping , restriction fragment length polymorphism , single nucleotide polymorphism , biology , genetics , polymerase chain reaction , allele , allele frequency , pyrosequencing , polymorphism (computer science) , defensin , gene , microbiology and biotechnology , genotype

Summary Polymerase chain reaction–restriction fragment length polymorphism assays for two single nucleotide polymorphisms in the human β‐defensin 1 gene have been validated with real‐time PCR in 101 healthy individuals from western Mexico. Allele frequencies were 52.5% (692‐A) and 98.5% (1836‐A). These assays can be confidently used as a cheaper alternative genotyping method for these sites.

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