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An accurate, high‐throughput method for genotyping the −373AnTn polymorphism in the interleukin‐6 promoter
Author(s) -
Khwaja Haris A.,
Green Fiona R.
Publication year - 2006
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2006.00569.x
Subject(s) - genotyping , haplotype , promoter , biology , capillary electrophoresis , genetics , microbiology and biotechnology , polymorphism (computer science) , gene , cytokine , genotype , computational biology , gene expression
Summary Interleukin‐6 (IL‐6) is a pro‐inflammatory cytokine implicated in inflammatory diseases. The four promoter polymorphisms (−174G/C, −373AnTn, −572G/C, −597G/A) have been shown to function as a haplotype in determining IL‐6 gene transcription. Using a capillary‐based electrophoresis system, high‐throughput genotyping for the −373AnTn polymorphism was achieved, thus facilitating IL‐6 promoter haplotype determination.