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Lack of association between the TNF‐α promoter gene polymorphism and susceptibility to B‐cell chronic lymphocytic leukaemia
Author(s) -
BoguniaKubik K.,
Mazur G.,
Urbanowicz I.,
Wróbel T.,
Kuliczkowski K.,
Woźniak M.,
Lange A.
Publication year - 2006
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.2005.00553.x
Subject(s) - single nucleotide polymorphism , allele , biology , tumor necrosis factor alpha , gene , snp , chronic lymphocytic leukemia , genetics , microbiology and biotechnology , polymerase chain reaction , immunology , b cell , cancer research , genotype , antibody , leukemia
Summary B‐cell chronic lymphocytic leukaemia (B‐CLL) is a lymphoproliferative disorder characterized by clonal expansion of B lymphocytes. The present study aimed to determine whether there is an association between the polymorphic features located within the promoter/enhancer region of tumour necrosis factor‐α ( TNFA ) gene and susceptibility to B‐CLL. TNFA (−308 G/A) promoter single nucleotide polymorphism (SNP) was determined by polymerase chain reaction with sequence‐specific primers (PCR‐SSP) using commercial oligonucleotides. No significant association was found between the distribution of TNFA alleles and B‐CLL in Polish patients with B‐CLL. Our single centre results were compared with other literature data and combined in a cumulative analysis employing the Mantel–Haenszel method. Among 183 B‐CLL patients, 47 (26%) were carrying TNFA*2 allele and this allele was present in 98 out of 348 controls (28%). Also, the results of the Mantel–Haenszel test did not show a significant correlation [Mantel‐Haenszel estimate of approximate relative risk (RMH) = 0.86, P = 0.294]. These results suggest that TNFA ( – 308) alleles are not involved in the predisposition to the development of B‐CLL.