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DETERMINATION OF DRB ALLELES USING PCR AMPLIFICATION AND ALLELE‐SPECIFIC PRIMERS
Author(s) -
Lepage V.,
Schaeffer V.,
Mallet C.,
Ivanova R.,
I. Khalil,
Charron D.
Publication year - 1994
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1994.tb00175.x
Subject(s) - polymerase chain reaction , allele , restriction fragment length polymorphism , biology , typing , variants of pcr , genetics , microbiology and biotechnology , gene
SUMMARY HLA class‐II allelic diversity is commonly defined using polymerase chain reaction (PCR) in combination with sequence‐specific oligotyping (PCR‐SSO) or the combination of PCR and restriction fragment length polymorphism methods (PCR‐RFLP). Nevertheless, the identification of the DRB polymorphism by PCR‐SSO is a time‐consuming procedure and the PCR‐RFLP is cumbersome. A rapid technique which allows a precise and extensive HLA‐DRB typing is required, particularly in order to study the role of class‐II matching in organ transplantation. A DRB typing method based on the detection and length of PCR products amplified using combination of allele specific primers has been developed. Thirty‐four DRB alleles (29 DRB1, 4DRB3, 1DRB4) can be detected using 29 primers distributed into 19 amplification mixtures.