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T‐CELL RECEPTOR α, δ, AND γ CHAIN GENES IN INSULIN‐DEPENDENT DIABETES MELLITUS
Author(s) -
MartianezNaves E.,
Penta M.,
LoapezLarrea C.
Publication year - 1993
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1993.tb00151.x
Subject(s) - restriction fragment length polymorphism , allele , haplotype , restriction enzyme , genotype , biology , restriction fragment , genetics , t cell receptor , microbiology and biotechnology , gene , t cell , immune system
SUMMARY We have studied the genotypic, haplotypic, and allelic distribution of germline Restriction Fragment Length Polymorphism (RFLP) of T‐cell receptor (Tcr) α, γ, and δ loci in 75 insulin‐dependent diabetes mellitus (IDDM) patients and 84 healthy blood donors as control population. The restriction endonuclease PvuII produces three allelic fragments of Tcr C‐γ (TcrCG) gene segment of 16,13, and 11.3 Kb respectively. Our observations revealed that Pvu II/TcrCG RFLP allelic distributions were not significantly different in the IDDM and the control group. However, 85% of IDDM patients carried HLA DR3 and/or DR4 haplotypes, and when comparing these patients with a second group of HLA DR3+ and/or DR4+ healthy individuals, the 11Kb/ Pvu II fragment of TcrCG gene was found to be associated with IDDM patients (χ= 11.4, P = 0.003). 54.9% of IDDM patients carried at least one 11.3 Kb allele vs. 21% in controls (χ= 10.77, P = 0.004). No significant association was found between RFLP in Tcr, Cα, Cδ, Vγ9 loci and IDDM.