Premium
FINE ANALYSIS OF RECOGNITION SITES OF MONOCLONAL ANTIBODIES, 1B7 AND 1E4, USING L CELL TRANSFECTANTS OF RECOMBINANT MOUSE MHC CLASS II GENES
Author(s) -
Itoh Y.,
Ogasawara K.,
Onoé K.
Publication year - 1993
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1993.tb00092.x
Subject(s) - monoclonal antibody , recombinant dna , biology , gene , major histocompatibility complex , class (philosophy) , genetics , microbiology and biotechnology , antibody , computer science , artificial intelligence
SUMMARY Recognition sites of two monoclonal antibodies (mAbs), 1B7 and 1E4, specific for murine MHC class II were determined, using eleven L cell transfectants which express recombinant class II (I‐A) molecules. 1B7 positive cells possessed a haplotype k in the α‐helix of the β‐chain, whereas 1E4 positive cells had haplotype b at the α‐helix of the β‐chain. The α‐helix of the β‐chains is regarded as an important site for antigen binding. Thus, the 1B7 and 1E4 mAbs may be useful for analysis of functional sites on class II molecules involved in antigen recognition.