Premium
DAMAGE IN B2m GENES AND DNA METHYLATION OF H‐2 GENES ARE INVOLVED IN LOSS OF EXPRESSION OF CLASS I MHC PRODUCTS ON THE MEMBRANE OF LR.4, A CELL LINE DERIVATIVE OF THE T‐CELL LYMPHOMA L5178Y
Author(s) -
Nava G.,
Ocadiz R.,
Ortega V.,
Alfaro G.
Publication year - 1992
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1992.tb00053.x
Subject(s) - biology , microbiology and biotechnology , gene , cytotoxic t cell , polyclonal antibodies , genomic dna , antiserum , antibody , antigen , major histocompatibility complex , immunogenetics , genetics , in vitro
SUMMARY We have isolated an H‐2 deficient cell line (LR.4) from the T‐cell lymphoma L5178Y which grew without restrictions in the peritoneal cavity of different inbred strains of mice. The use of polyclonal anti‐H‐2 antiserum and complement indicated that LR.4 cells did not express class I determinants on the cell membrane. Southern blots of genomic DNA of LR.4 cells showed that B2m genes were severely damaged and that class I H‐2 genes were extensively methylated. Consequently, LR.4 cells failed to transcribe mRNAs for both B2m and class I H‐2 genes. On the other hand, specific immunity to LR.4 was demonstrated in C57BL/6J mice since, in subsequent challenges with either LR.4 or EL4.4, LR.4 did not grow, whereas EL4.4 grew and killed the mice. In C57BL/6J mice, rejection of LR.4 was accompanied by the production of cytotoxic antibodies. The immune response induced in C57BL/6J mice was determined by non‐H‐2 antigenic determinants in LR.4 cells.