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HETEROGENEOUS EXPRESSION OF BLOOD GROUP A‐DETERMINANT IN A HUMAN GASTRIC CANCER CELL LINE DERIVED FROM A BLOOD GROUP A INDIVIDUAL
Author(s) -
Kominato Y.,
Fujikura T.,
Takizawa H.,
Hayashi K.,
Fujimaki M.,
Kishi K.,
Suzuki T.
Publication year - 1992
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1992.tb00042.x
Subject(s) - antigen , mutant , biology , cell culture , immunofluorescence , microbiology and biotechnology , cell , cancer , cancer cell , gene , antibody , immunology , cancer research , genetics
SUMMARY The human gastric cancer cell line MKN 45 was derived from the tumour of a blood group A individual, and was known to express large quantities of blood group A‐antigen. Using immunofluorescence we found the MKN 45 cells, donated from the Japanese Cancer Research Resources Bank, consisted of A‐antigen positive cells (18%) and A‐antigen negative cells (82%). After limiting dilution, wild type and mutant cells were cloned with regard to the expression of a cell surface A‐antigen. ELISA was used to detect A‐antigen in the cell extract of the wild type cells, but none was evident in those of the mutant cells. However, blood group A‐gene‐specified transferase activity of the mutant cells was comparable to that of the wild type cells.