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H‐2 INFLUENCE ON THE PRODUCTION OF REAL BUT NOT OF APPARENT H‐2‐SPECIFIC ANTIBODIES INDUCED BY THE ASSOCIATION OF SYNGENEIC CLASS I HEAVY CHAINS WITH BOVINE β2‐MICROGLOBULIN
Author(s) -
Rocca A.,
Samaan A.,
Michel C.,
Besluau D.,
Degos L.,
Pla M.
Publication year - 1991
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1991.tb00036.x
Subject(s) - antibody , beta 2 microglobulin , microbiology and biotechnology , beta (programming language) , lymphoblast , chemistry , immunoglobulin light chain , biology , immunology , cell culture , genetics , computer science , programming language
SUMMARY Immunogenic properties of class I molecules resulting from the association of mouse class I heavy chains with a xenogeneic β2‐microglobulin (β2‐m) were investigated by studying the antibody response of mice of injections to syngeneic Con A lymphoblasts, induced in conditions allowing the replacement of endogenous β2‐m by exogenously added bovine (β2‐m provided by fetal calf serum (FCS‐Con A blasts). Lymphocyto‐toxic antibodies were regularly produced and according to their specificities they could be divided into two types: antibodies whose reactivity was (1) dependent on and (2) independent of the presence of bovine β2‐m on target cells. Although both types displayed an H‐2 dependent polymorphic reaction pattern, only antibodies recognizing class I molecules without bovine β2‐m can be considered as real H‐2‐specific antibodies. The others are only apparent H‐2‐specific antibodies: their polymorphic reaction pattern is dependent both on the presence of bovine β2‐m on the surface of target cells and on their H‐2 haplotype. A comparison of the antibody response of mice with various H‐2 haplotypes to injections of syngeneic FCS‐Con A blasts showed no significant difference in the induction of bovine β2‐m‐dependent antibodies (apparent H‐2‐specific) among the mice from all strains tested (H‐2 b , H‐2 p , H‐2 q , and H‐2 S ). Unexpectedly, for most strains more than 60% of the immunized mice produced also β2‐m‐independent antibodies (real H‐2‐specific), with the exception of H‐2 q mice, in which only 30% of sera were positive. The real H‐2‐specific antibody response is of two types: some mice (H‐2 P and H‐2 S ) produced antibodies only reactive with allogeneic target cells whereas others (H‐2 b and H‐2 q ) produced in addition antibodies that were reactive with syngeneic cells. Thus H‐2 appears to play an important role in the induction and specificity of the lymphocytotoxic H‐2‐specific antibodies induced upon immunization with cells expressing syngeneic class I heavy chains associated with bovine β2‐m.