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ANALYSIS OF Ly5 CHROMOSOME 1 POSITION USING ALLELIC DIFFERENCES AND RECOMBINANT INBRED MICE
Author(s) -
Zebedee S.L.,
Barritt D.,
Epstein R.,
Raschke W.C.
Publication year - 1991
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1991.tb00015.x
Subject(s) - biology , inbred strain , recombinant dna , microbiology and biotechnology , genetics , gene , allele
SUMMARY Differences between the mouse Ly5 a and Ly5 b alleles can be distinguished on the basis of polymerase chain reaction (PCR)‐restriction enzyme analysis and differential monoclonal antibody reactivities. To more precisely map the Ly5 gene on the mouse chromosome 1, analytical DNA and protein tests were performed on recombinant inbred strains of mice prepared from SJL/J ( Ly5 a ) and B ALB/cke ( Ly5 b ) progenitor strains. Each recombinant inbred strain was characterized to determine whether it carried the Ly5 a or Ly5 b allele. Both assays, DNA‐PCR and protein‐immunofluorescence, yielded identical results for each strain examined. Placement of the Ly5 gene with respect to other characterized markers of mouse chromosome 1 for these recombinant inbred mouse strains shows a gene order of Idh‐1.Ity:Pep3: / Ly5, CfhJ.