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HLA‐DR/Dw MATCHING BY PCR FINGERPRINTING: THE ORIGIN OF PCR FINGERPRINTS AND FURTHER APPLICATIONS
Author(s) -
Wood N.A.P.,
Clay T.M.,
Bidwell J.L.
Publication year - 1991
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1991.tb00014.x
Subject(s) - heteroduplex , dna profiling , primer (cosmetics) , polymerase chain reaction , microbiology and biotechnology , biology , genetics , dna , fingerprint (computing) , gene , chemistry , computer security , organic chemistry , computer science
SUMMARY Polymerase chain reaction (PCR) fingerprinting, a new method for the rapid matching of HLA‐Dr/Dw allotypes, involves the visual comparison of polymorphic HLA‐DRB gene second exon PCR products, resolved in non‐denaturing polyacrylamide gels (Bidwell & Hui, 1990). We show here that the satellite DNA bands within PCR fingerprints originate by heteroduplex formation between heterologous DNAs co‐amplified by a common PCR primer set. We also present two further applications of the technique which permit discrimination between unrelated HLA‐DR/Dw allotypes with similar PCR fingerprints.