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PCR‐SSO TYPING IN HLA‐DISEASE ASSOCIATION STUDIES
Author(s) -
WORDSWORTH P.
Publication year - 1991
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1991.tb00013.x
Subject(s) - major histocompatibility complex , human leukocyte antigen , immunology , typing , biology , allele , genetics , rheumatoid arthritis , population , autoimmune disease , disease , histocompatibility , antigen , gene , medicine , antibody , pathology , environmental health
SUMMARY Associations between a large number of diseases and markers within the major histocompatibility complex (MHC) have been described. In particular, susceptibility to several autoimmune disorders, including type I diabetes mellitus and rheumatoid arthritis, is linked to genes within the MHC and strong population associations are demonstrable between certain HLA class II alleles and these conditions. Genetic mapping of HLA susceptibility loci has traditionally relied on the use of phenotypic markers defined by alloantisera, cellular typing reagents and biochemical analysis of histocompatibility antigens. Polymerase chain reaction sequence‐specific oligonucleotide (PCR‐SSO) typing combines the ability to define the finest of HLA specificities, by analysis of the corresponding DN A sequences, with the possibility of studying large populations of normal and affected individuals. The applications of this technology to characterizing precisely the MHC loci associated with susceptibility to autoimmune diseases such as rheumatoid arthritis, type I diabetes mellitus, coeliac disease and pemphigus vulgaris are reviewed here.