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DISCRIMINATION BETWEEN HLA‐DR1 (DRB1*0101) AND DR'Br’(DRB1*0103) USING SEQUENCE‐SPECIFIC OLIGONUCLEOTIDE PROBES
Author(s) -
Bidwell J.L.,
Bidwell E.A.
Publication year - 1991
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1991.tb00010.x
Subject(s) - oligonucleotide , haplotype , restriction fragment length polymorphism , genetics , biology , allele , oligomer restriction , human leukocyte antigen , identification (biology) , microbiology and biotechnology , polymorphism (computer science) , gene , polymerase chain reaction , antigen , botany
SUMMARY Serological identification of the HLA‐DQw1(w5)‐associated or HLA‐DQw3(w7)‐associated DR ‘Br’(DRB1*0103) allele cannot be accomplished in the presence of a second DQw1(w5)‐positive or DQw3(w7)‐positive haplotype, respectively. DNA‐restriction fragment length polymorphism (RFLP) analysis assists in identification of DR ‘Br’, though not in the presence of DR1. We describe an alternative or complementary method for identification of DR ‘Br’ using two oligonucleotide probes which target HLA‐DRB1 gene HV3 regions.