Premium
PCR‐SSO TYPING FOR DR4‐Dw SUBTYPES: APPLICATION TO UNRELATED BONE MARROW TRANSPLANT DONOR SELECTION
Author(s) -
Clay T. M.,
Howard M. R.,
Bidwell J. L.,
Bidwell E. A.,
Raymond P. A.,
Evans J. E.,
Bradley B. A.
Publication year - 1991
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1991.tb00009.x
Subject(s) - subtyping , typing , restriction fragment length polymorphism , oligonucleotide , bone marrow , bone marrow transplantation , bone marrow transplant , biology , dna , medicine , computational biology , polymerase chain reaction , microbiology and biotechnology , genetics , immunology , computer science , gene , programming language
SUMMARY Thirty‐seven DR4‐positive patient‐unrelated bone marrow donor pairs previously DR/DQ restriction fragment length polymorphism (RFLP) typed and tested in mixed lymphocyte culture (MLC), have been DR4‐Dw subtyped retrospectively using sequence specific oligonucleotide probes. We found that DR4‐Dw subtyping substantially increased the accuracy of pre‐MLC matching and could potentially accelerate donor searches by avoiding unnecessary MLC tests on Dw‐mismatched donors.