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V H DJ H TO J H JOINING IS NOT BLOCKED IN μ‐CHAIN‐PRODUCING PRE‐B CELLS, IMPLYING THE BREAKDOWN OF ALLELIC EXCLUSION
Author(s) -
Sugiyama H.,
Komori T.,
Minami Y.,
Sakato N.
Publication year - 1990
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1990.tb00891.x
Subject(s) - chain (unit) , allelic exclusion , allele , microbiology and biotechnology , physics , genetics , biology , gene , t cell , astronomy , t cell receptor , immune system
SUMMARY Intracytoplasmic μ‐positive pre‐B cells (μ + V H 315 − cells) transformed with Abelson virus continuously produced cells (μ + V H 315 + cells) that were double‐stained by anti‐μ and anti‐V H 315 antibodies which specifically recognized the immunoglobulin heavy chain variable region identical or closely related to that of MOPC315 myeloma protein. Southern blot analysis indicated that the μ + V H 315 + cells were generated from the μ + V H 315‐ cells by the V H 315·D·J H2 to a germline J H3 joining on the non‐productive V H 315·D·J H2 allele, which resulted in the production of μ‐chains with variable region detectable by anti‐V H 315 antibodies. Therefore, it is strongly indicated that V H DJ H to J H joining is not blocked in μ‐chain‐producing pre‐B cells, and thus is free from allelic exclusion machinery.