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CHARACTERIZATION OF SOLUBLE HLA MOLECULES IN SWEAT AND QUANTITATIVE HLA DIFFERENCES IN SERUM OF HEALTHY INDIVIDUALS
Author(s) -
Zavazava N.,
Westphal E.,
MullerRuchholtz W.
Publication year - 1990
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1990.tb00890.x
Subject(s) - human leukocyte antigen , sweat , isoelectric focusing , chemistry , monoclonal antibody , microbiology and biotechnology , isoelectric point , antibody , antigen , biology , biochemistry , immunology , enzyme , medicine
SUMMARY Soluble class I molecules were immunoprecipitated from human sweat and serum using the BB7.7 monoclonal antibody (mAb) coupled to immunomagnetic beads. Molecules were analysed biochemically on SDS‐PAGE gels and finally by 1D‐isoelectric‐focusing (IEF). Serum‐ and sweat‐HLA IEF‐band patterns of the same individual were fully identical, showing that HLA excreted in sweat possess polymorphic structures like those in serum. Quantitatively, we used a highly sensitive competitive enzyme‐linked immunosorbent (ELISA) assay to determine soluble class I concentrations. The first group was that of non‐HLA‐A9 and ‐Bw62 sera, which were found to contain HLA levels with a mean concentration of 0.82 ± 0.63 μg/ml ( n = 44). However, sera that were HLA‐A23 or −24 (splits of HLA‐A9) contained higher levels, with a mean of 3.2 ± 0.94 μg/ml ( n = 20). Similarly, HLA‐Bw62 individuals had a higher mean of 2.05 ± 0.65 μg/ml ( n = 10). The difference of the HLA‐A9 group to the first group was statistically highly significant, P < 0.0001, and that of the HLA‐Bw62 to the first was also significant, P < 0.004. Individuals who were both HLA‐A9 and ‐Bw62 ( n = 5) did not express significantly higher levels than those who only had one of these specificities. Sweat HLA levels had a mean of 0.42 ± 0.4 μg/ml ( n = 10). These results show for the first time that soluble class I peptides are excreted in relatively high concentrations in sweat and possess polymorphic structures identical to those of serum HLA and that serum HLA levels are allotype dependent.