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MOLECULAR ANALYSIS OF TWO RECOMBINANT MOUSE STRAINS WITH CROSSOVERS IN THE E α RECOMBINATION HOT SPOT
Author(s) -
Lafuse W. P.,
Lee S. T.,
David C. S.
Publication year - 1990
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1990.tb00869.x
Subject(s) - recombinant dna , recombination , gene , genetics , biology , microbiology and biotechnology , haplotype , restriction fragment length polymorphism , genetic recombination , genotype
SUMMARY Recombination in the E α gene of the mouse major histocompatibility complex has been found to occur only in mice derived from crosses between strains with the k and p haplotypes. In the present paper the crossover sites of six additional strains were examined by RFLP analysis. These recombinant strains were derived from crosses between strains (B10.RPD1, B10.RPD2) with crossovers in the E α gene and B10.M ( H‐2 f ). Four have crossover sites between the E α gene and the S region. One recombinant (B10.RFD7) also crossed over within the E α gene and a second (B10.RPF1) crossed over within a segment located to the left of the E α gene. This study suggests that the DNA sequence responsible for recombination in the E α gene is also present in the B10.RPD1 and B10.RPD2 recombinant strains.