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RADIATION LEUKEMIA VIRUS AND ITS EFFECT ON H‐2 GENE EXPRESSION
Author(s) -
Brown G. D.,
Meruelo D.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00482.x
Subject(s) - leukemia , gene , biology , gene expression , expression (computer science) , virus , virology , genetics , cancer research , computer science , programming language
SUMMARY In this report we demonstrate that lowered expression of the H‐2 antigens on RadLV‐induced tumour cells is a result of depressed levels of stable mRNA in these cells. Whether this observation is a result of lowered transcription or of mRNA instability is under investigation. In an effort to determine which viral sequences are essential for mediating both the H‐2 regulatory function and the transforming function of RadLV, we have begun to assemble newly integrated proviral genomes from tumours. The restriction enzyme cleavage sites of four isolates are presented; these isolates differ substantially from RadLV genomes previously presented. One of these molecular clones is shown to encode a non‐defective B‐tropic, ecotropic virus which when reinjected into resistant mouse strains can mediate the up‐regulation of H‐2D d antigen expression. Finally, possible mechanisms of H‐2 regulation are discussed.