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CLONING AND CHARACTERIZATION OF A POLYMORPHIC CLASS I MHC GENE IN THE AKR LYMPHOMA K36.16
Author(s) -
Sim B. C.,
Hui K. M.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00480.x
Subject(s) - biology , gene , microbiology and biotechnology , major histocompatibility complex , genetics , genomic dna , coding region , somatic cell , mhc class i , dna
SUMMARY Cancers are the result of somatic heritable changes in certain genes. The AKR leukaemia K36.16 has been extensively studied in our laboratory. When compared to normal AKR thymocytes, the K36.16 tumour cells do not express the H‐2K k antigens and have an unexpected antigenic determinant that could be detected by anti‐H‐2D d monoclonal antibodies. To understand the molecular mechanisms that could be responsible for these changes, we have compared the genomic composition of the class I MHC genes in the K36.16 tumour cells to that of normal AKR lymphocytes. A unique polymorphic 2.6‐kb Hind III fragment was detected in DNA obtained from the K36.16 tumour cells after hybridization with a 3′‐gene‐coding H‐2 probe. This fragment is not present in DNA of normal AKR lymphocytes. In an effort to further understand the mechanism underlying the nature of this MHC gene polymorphism, we have cloned and sequenced this Hind III fragment. When compared with the reported sequences of a number of mouse class I MHC genes, the nucleotide sequence of this polymorphic Hind III fragment is similar to that of a reported Tla gene.