Premium
mAb KUL/05 IDENTIFIES A DENATURATION‐RESISTANT DETERMINANT SHARED BY CLASS II MHC PRODUCTS DR, DQ AND DP
Author(s) -
Giacomini P.,
Tecce R.,
Nicotra M. R.,
Cohen B. B.,
Mazzilli M. C.,
Natali P. G.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00463.x
Subject(s) - monoclonal antibody , microbiology and biotechnology , major histocompatibility complex , mhc class ii , antigen , chemistry , mhc class i , biology , mutant , antibody , immunology , biochemistry , gene
SUMMARY mAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα‐ mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specifities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain‐associated forms, and (c) reacts with frozen, acetone‐fixed, as well as conventional, formalin‐fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue specimens.