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THREE ANTIBODIES TO HUMAN INTESTINAL BRUSH BORDER MEMBRANE ARE CHARACTERIZED AS ANTI‐Le a
Author(s) -
Dickson L.,
Richman P. I.,
Shaw M.A.,
Donald A. S. R.,
Swallow D. M.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00462.x
Subject(s) - glycoprotein , antibody , monoclonal antibody , epitope , microbiology and biotechnology , brush border , biochemistry , chemistry , agglutination (biology) , biology , immunology , membrane , vesicle
SUMMARY In the course of raising monoclonal antibodies to human colonic and small intestinal epithelium, three were isolated which recognized epitopes on many colonic glycoproteins and many jejunal glycoproteins taken from some individuals, but not on the same glycoproteins from others. The specificities of these antibodies were examined by carrying out inhibitions of the binding of the antibodies to the jejunal glycoproteins by blood group substances and purified oligosaccharides. The glycoproteins were separated by electrophoresis and transferred to nitrocellulose strips which were then used for the inhibition experiments. The results indicated that the antibodies bind to Le a ‐active structures. The conditions were determined for achieving red cell agglutination by two of the antibodies.