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ISOLATION OF EPIDERMAL LANGERHANS CELLS
Author(s) -
Schmitt D. A.,
Hanau D.,
Cazenave J.P.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00458.x
Subject(s) - immunomagnetic separation , panning (audio) , centrifugation , differential centrifugation , isolation (microbiology) , langerhans cell , density gradient , antigen , immune system , microbiology and biotechnology , cell , biology , bone marrow , immunology , biochemistry , bioinformatics , paleontology , zoom , physics , quantum mechanics , lens (geology)
SUMMARY Langerhans cells (LC) play an important role in the skin immune system. They are bone marrow‐derived and function as the only accessory and antigen‐presenting cells in the skin. Several techniques for enriching these cells have been devised, and four, including density gradient centrifugation, use of cell sorter, panning and immunomagnetic separation, are discussed. It is concluded that the most satisfactory method for isolation of LC is based on density gradient centrifugation and the most satisfactory for depletion of epidermal cell preparations for LC is based on the immunomagnetic principle.