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DIRECT HLA TYPING BY ROSETTING WITH IMMUNOMAGNETIC BEADS COATED WITH SPECIFIC ANTIBODIES
Author(s) -
Hansen T.,
Hannestad K.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00455.x
Subject(s) - human leukocyte antigen , rosette (schizont appearance) , typing , monoclonal antibody , antibody , tissue typing , microbiology and biotechnology , biology , chemistry , antigen , immunology , genetics
SUMMARY Paramagnetic monodisperse polymer beads were coated with purified human hybridoma antibodies specific for polymorphic HLA determinants. The mAb‐coated beads bound specifically to cells from individuals with the relevant HLA type. The rosettes formed by beads and cells were isolated with a magnet. Rosette formation was evaluated microscopically and used as criterion for positive typing. By this rosette assay, fast and reliable HLA typing of whole blood was possible.