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SPECIFICITY OF ANTI‐H‐2 CLASS I ANTIBODIES INDUCED BY SYNGENEIC IMMUNIZATION WITH SENDAI VIRUS‐TREATED CELLS IS REGULATED BY THE MOUSE MHC AND VIRAL ANTIGENS. NO EVIDENCE FOR MHC‐RESTRICTED VIRUS‐ SPECIFIC ANTIBODIES
Author(s) -
Kievits F.,
Van Bleek G. M.,
Boerenkamp W. J.,
Pla M.,
Ivanyi P.
Publication year - 1989
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1989.tb00443.x
Subject(s) - sendai virus , antibody , virology , biology , major histocompatibility complex , antigen , virus , immunization , immune system , mhc class i , immunology
SUMMARY In a previous study, we searched for Sendai virus (SV)‐specific antibodies that were restricted in their binding by self‐major histocompatability complex (MHC) antigens. In C57BL/6 (B6; H‐2 b ) mice, most of the sera obtained after i.p. injections with syngeneic SV‐coated (SV + ) spleen cells contained auto‐ and alloreactive lymphocytotoxic antibodies directed against H‐2 class I molecules, but no viral‐specific, MHC‐restricted antibodies. Here we report that syngeneic immunization with SV + cells regularly induced H‐2‐specific antibodies in various mouse strains. From a total of 12 strains tested, only the B10.S ( H‐2 s ) strain appeared to be a low responder. The immune responses are of two types: (i) mice of some strains produce autoreactive antibodies and a broad variety of alloreactive antibodies; and (ii) mice of some strains produce only narrow or widely alloreactive antibodies. Because most of the strains differ only in the H‐2 region, the patterns observed are regulated by the MHC. To locate the genes involved in the induction of H‐2‐specific antibodies more precisely, two B6 mutant strains, bml ( K b mutant) and bm13 ( D b mutant), were immunized with syngeneic SV + cells. The results suggest that the H‐2D b region plays an important role in the induction and specificity of the lymphocytotoxic H‐2 class I‐specific antibodies present in sera of H‐2 b mice after syngeneic immunization with SV + cells.