Premium
H‐Y RESPONSES OF NON‐OBESE DIABETIC (NOD) MICE
Author(s) -
Chandler P.,
Fairchild S.,
Simpson E.
Publication year - 1988
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1988.tb00435.x
Subject(s) - nod , nod mice , medicine , diabetes mellitus , endocrinology
SUMMARY Female non‐obese diabetic (NOD) mice were tested for their ability to make responses to the male‐specific (H‐Y) transplantation antigen. In vivo assessment of this ability was made using skin graft rejection. A proportion (60%) spontaneously rejected NOD male tail skin by 80 days post‐transplantation. The detection of the generation of H‐Y‐specific cytotoxic T cells, following in vivo priming and secondary in vitro restimulation, was carried out using a conventional 51 Cr release assay. Female NOD mice primed either by skin grafting, intraperitoneal (i.p.) or footpad (f.p.) injection of male NOD spleen cells could be induced to make anti‐H‐Y cytotoxic responses, but not every immunized mouse responded. The nature of the H‐Y‐reactive T cells was investigated further by the in vitro isolation of T‐cell clones of which some were H‐Y specific.