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FURTHER SEROLOGICAL AND RFLP ANALYSIS OF THE MRL‐ +/+ AND MRL‐ lpr/lpr MICE
Author(s) -
DeLarbre C.,
Bobe P.,
Kiger N.,
Kourilsky P.,
Gachelin G.
Publication year - 1988
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1988.tb00434.x
Subject(s) - serology , major histocompatibility complex , epitope , immunology , restriction fragment length polymorphism , genotyping , haplotype , antigen , biology , mhc class i , gene , microbiology and biotechnology , genetics , antibody , genotype
SUMMARY During their ageing, MRL‐ lpr/lpr mice ( H‐2 k ) suffer from progressive lymph node enlargement, associated with the development of several acute autoimmune lesions. The primary effect and location of the lpr mutation is unknown. However, a minority of the lymphoid cells of some MRL‐ +/+ , as well as MRL‐ lpr/lpr mice, express ‘alien’ H‐2 d antigenic specificities. In the course of the investigation of the origin of the latter, we have carried out the genotyping of the MHC of several MRL‐ +/+ and MRL‐ lpr/lpr mice using the Southern blotting technique and employing a variety of MHC class I and class II probes, and restriction enzymes known to discriminate between the d and the k haplotypes. None of the results obtained so far suggests the contribution of genuine H‐2 d genes to the MHC of the MRL‐ +/+ and MRL‐ lpr/lpr mouse strains. Alternative hypotheses for the generation of the ‘alien’ epitopes are discussed.