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A MONOCLONAL ANTIBODY, R1, AND A POLYCLONAL SERUM, S10, RECOGNIZE THE SAME MOLECULES: A NOVEL USE OF DNA TRANSFECTANTS
Author(s) -
Banting G.,
Mondello C.,
Hope R.,
Goodfellow P.
Publication year - 1988
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1988.tb00429.x
Subject(s) - polyclonal antibodies , microbiology and biotechnology , phosphoprotein , immunoprecipitation , monoclonal antibody , antiserum , chemistry , dna , antibody , biology , biochemistry , gene , genetics
SUMMARY The monoclonal antibody R1 defines the product of the MIC5 locus which has been localized to the long arm of the human X chromosome in the same region as the fragile site FRAXA. DNA transfectants, selected by R1 on the fluorescence‐activated cell sorter (FACS), have been used to demonstrate that a previously described polyclonal antiserum, S10 (Buck & Bodmer, 1976), recognizes the same cell surface molecule as R1. Immunoprecipitation from metabolically and surface‐labelled cells has shown that this molecule is a M r 200 K phosphoprotein which is synthesized as a M r 180 K precursor and subsequently modified to a M r 195 K extracellular form. A M r 150 K molecule is coprecipitated with the M r 200 K phosphoprotein, but is only detected in lysates of surface‐labelled cells, raising the possibility that the 200 K molecule is a cell surface receptor and the 150 K molecule a ligand.