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EXPRESSION OF HUMAN CD4 BY TWO HUMAN‐MOUSE INTERLINEAGE HYBRIDS
Author(s) -
Taylor G. M.,
Morten J. E. N.,
Morten H.,
Dodge A. B.,
Rldway J. C.,
Jones P. M.,
Harris R.
Publication year - 1988
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1988.tb00422.x
Subject(s) - hybrid , expression (computer science) , biology , genetics , computer science , botany , programming language
SUMMARY Two hybrid cell lines expressing human CD4 were prepared by fusing human B‐lymphoid cells with the mouse T‐lymphoma BW5147. Hybrid TF42 was derived from a human B‐lymphoblastoid line and TF53.1 from a human B‐ALL. Variants of these hybrids expressing or lacking CD4 were isolated by sorting cells stained with the monoclonal antibody (mAb) OKT4 on a fluorescence‐activated cell sorter (FACS). Cytogenetic, isoenzyme and DNA analysis confirmed the presence of human chromosome 12 in the CD4 + hybrids, and revealed that CD4 expression by TF42 was associated with multiple copies of this chromosome. Of seventy mAb recognizing human T‐cell antigens screened on the CD4 + and CD4 − variants of the two hybrids, only mAb recognizing CD4 and Leu 8 reacted with the CD4 + cells. These hybrids should be useful in the preparation, screening and analysis of anti‐CD4 monoclonal antibodies, and in studies of CD4 epitopes recognized by HIV.