Premium
DNA SEQUENCE ANALYSIS OF TWO BOVINE IMMUNOGLOBULIN C H GAMMA PSEUDOGENES
Author(s) -
Symons D. B. A.,
Clarkson C. A.,
Milstein C. P.,
Brown N. R.,
Beale D.
Publication year - 1987
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1987.tb00392.x
Subject(s) - pseudogene , gene , biology , exon , intron , genetics , microbiology and biotechnology , homology (biology) , gene duplication , dna , bovine genome , sequence analysis , peptide sequence , genome
SUMMARY A bovine calf liver DNA library in λ2001 bacteriophage has been screened with a human Igγ4 heavy‐chain constant‐region gene probe. Four hybridizing clones have been identified, and the DNA sequences in two of these, which have high homology with C H γ genes, are reported here. Within the bovine sequences, four separate exons can be identified, corresponding to the three C H domains and the hinge of gamma heavy‐chain genes. Both of these genes contain atypical sequences around one or more of their exon/intron boundaries with consequent loss of splice sites, indicating that these are probably γ pseudogenes. One sequence codes for a C‐terminal peptide which matches the 18‐mer C‐terminal heavy‐chain peptide of bovine serum IgG2, the other encodes a C‐terminal peptide unknown in the bovine. These results suggest that evolutionary duplication of C H γ genes has occurred in the bovine.