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ANTIGEN‐SPECIFIC HELPER FACTOR REACTS WITH ANTIBODY TO THE T‐CELL RECEPTOR
Author(s) -
Deans J. P.,
Krowka J. F.,
Mosmannt T.,
Pilarski L. M.
Publication year - 1987
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1987.tb00369.x
Subject(s) - antigen , t cell receptor , monoclonal antibody , microbiology and biotechnology , cytotoxic t cell , biology , clone (java method) , ctl* , t lymphocyte , major histocompatibility complex , epitope , t cell , antibody , chemistry , immunology , biochemistry , immune system , cd8 , in vitro , dna
SUMMARY Antigen‐specific helper factor (ASHF), a soluble product of T helper (Th) cells, binds antigen and can induce B‐cell and cytotoxic T‐lymphocyte (CTL) differentiation. Its relationship to the T‐cell surface antigen receptor (TcR) is unknown. Both have MHC‐restricted recognition of nominal antigen, thus they may share very similar combining sites. Using monoclonal anti‐TcR to imrnunoprecipitate partially purified ASHF, we have obtained evidence for shared determinants between ASHF and the TcR. Antigen affinity‐enriched supernatants of a Th clone, LB19, are functionally active in antigen‐specific, help‐dependent CTL assays. FPLC anion exchange salt fractions of these supernatants were 125 I‐labelled and immunoprecipitated with KJ16.133 monoclonal anti‐TcR coupled to Sepharose 4B. Precipitates were analysed by SDS‐PAGE. We have obtained clear evidence that functionally active Th culture supernatants contain molecules specifically precipitable by anti‐TcR antibody.