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MOLECULAR COMPLEXITY OF Qa‐2 ANTIGENS DEMONSTRATED BY TWO‐DIMENSIONAL GEL ELECTROPHORESIS
Author(s) -
Robinson P. J.,
Lefkovits I.,
Lindahl K. Fischer
Publication year - 1987
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1987.tb00366.x
Subject(s) - antiserum , congenic , microbiology and biotechnology , antigen , biology , immunoprecipitation , gel electrophoresis , labelling , electrophoresis , phenotype , polyacrylamide gel electrophoresis , cell culture , biochemistry , gene , genetics , enzyme
SUMMARY Biosynthetically labelled Qa‐2 antigens were isolated from mouse spleen cells by immunoprecipitation with anti‐Qa‐2 antisera. When newly synthesized Qa‐2 molecules from several different inbred strains were analysed by two‐dimensional (2D) gel electrophoresis; four different phenotypes were observed that differed in the number of polypeptides present. The ability to distinguish Qa‐2 + phenotypes was used to map the recombination points in two congenic strains, B6. Tla a and A. Tla b . No alternative Qa‐2‐like polypeptides were detected in B6.K1 (Qa‐2 − ) cells using a polyspecific rabbit antiserum against mouse class I antigens, but a new molecule was detected in BALB/cBy (Qa‐2 − ) cells. Pulse‐chase and surface‐labelling experiments showed that some, but not all, of the newly synthesized Qa‐2 precursor forms are processed to mature cell surface molecules.