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ABROGATION OF LETHAL GRAFT‐VERSUS‐HOST REACTION DIRECTED AGAINST NON‐H‐2 ANTIGENS: ROLE OF Mls a AND K/I REGION ANTIGENS IN THE INDUCTION OF UNRESPONSIVENESS BY ALLOIMMUNIZATION
Author(s) -
HallePannenko O.,
Pritchard L. L.,
Festenstein H.,
Berumen L.
Publication year - 1986
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1986.tb01128.x
Subject(s) - antigen , host (biology) , biology , immunology , virology , genetics
SUMMARY A graft‐versus‐host reaction (GVHR) directed against DBA/2 non‐H‐2 antigens alone can be induced by grafting B10.D2 bone marrow and spleen cells intravenously to heavily irradiated, H‐2 d compatible (DBA/2 x B10.D2)F 1 adult mice. Under the experimental conditions used, only 0‐l0% of recipients survive, but the survival is greatly increased by donor alloimmunization, a few days prior to grafting, against host‐specific (DBA/2) non‐H‐2 antigens and non‐specific (foreign) H‐2 antigens. The increased survival is mediated by alloimmunization‐activated suppressor cells which can decrease the intensity of the immune reaction developed by normal B10.D2 cells both in vivo (GVHR) and in vitro (proliferative response measured in mixed lymphocyte culture, MLC). The present experiments were designed to explore the antigenic requirements for inducing suppression. The results showed that in GVHR the protective effect induced by donor alloimmunization against the specific non‐H‐2 antigens, which leads to 70‐80% survival, is due primarily, if not entirely, to immunization against Mls 2 antigens. Results of MLC experiments confirmed this conclusion, showing that immunization against Mls a antigens is sufficient to account for the suppressive effect induced by the specific immunization. In addition, they indicated that the nonspecific protective effect induced by donor alloimmunization against foreign H‐2 antigens, which leads to 20‐30% survival, is due to immunization against antigens encoded by the K and/or I region(s) of the H‐2 complex; immunization against D region encoded antigens alone has no effect.